Journal article

The oppD Gene and Putative Peptidase Genes May Be Required for Virulence in Mycoplasma gallisepticum

Chi-Wen Tseng, Chien-Ju Chiu, Anna Kanci, Christine Citti, Renate Rosengarten, Glenn F Browning, Philip F Markham

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 2017


Relatively few virulence genes have been identified in pathogenic mycoplasmas, so we used signature-tagged mutagenesis to identify mutants of the avian pathogen Mycoplasma gallisepticum with a reduced capacity to persist in vivo and compared the levels of virulence of selected mutants in experimentally infected chickens. Four mutants had insertions in one of the two incomplete oppABCDF operons, and a further three had insertions in distinct hypothetical genes, two containing peptidase motifs and one containing a member of a gene family. The three hypothetical gene mutants and the two with insertions in oppD1 were used to infect chickens, and all five were shown to have a reduced capacity to ..

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