An investigation of gene expression in single cells derived from Nestin-expressing cells in the adult mouse midbrain in vivo
Parisa Farzanehfar, Malcolm K Home, Tim D Aumann
NEUROSCIENCE LETTERS | ELSEVIER IRELAND LTD | Published : 2017
Generation of new dopamine (DA) neurons in the adult midbrain is a controversial issue in development of better treatments for Parkinson's disease (PD). Previous research suggests Nestin-expressing neural precursor cells (NPCs) have a propensity to differentiate into neurons here, including DA neurons. In the present study we sought confirmation of this by studying gene expression in single Nestin-expressing cells and their progeny/ontogeny within the adult mouse midbrain. Cells were identified by administering a pulse of Tamoxifen to adult Nestin-CreERT2×R26eYFP transgenic mice. Samples of cytoplasm were harvested 4 days to 8 months later from individual eYFP+ cells in acutely prepared midb..View full abstract
The authors acknowledge important contributions to this study from Dr Anupa Dey, Dr Elena Gazina and Professor Leonid Churilov. The work was supported by a grant from the National Health and Medical Research Council of Australia.