Journal article
Identification of genetic markers of resistance to echinocandins, azoles and 5-fluorocytosine in Candida glabrata by next-generation sequencing: a feasibility study
C Biswas, SCA Chen, C Halliday, K Kennedy, EG Playford, DJ Marriott, MA Slavin, TC Sorrell, V Sintchenko
Clinical Microbiology and Infection | Published : 2017
Abstract
Objectives Multi-antifungal drug resistance in Candida glabrata is increasing. We examined the feasibility of next-generation sequencing (NGS) to investigate the presence of antifungal drug resistance markers in C. glabrata. Methods The antifungal susceptibility of 12 clinical isolates and one ATCC strain of C. glabrata was determined using the Sensititre YeastOne® YO10 assay. These included three isolate pairs where the second isolate of each pair had developed a rise in drug MICs. Single nucleotide polymorphisms (SNPs) in genes known to be linked to echinocandin, azole and 5-fluorocytosine resistance were analysed in all isolates through NGS. Results High-quality non-synonymous SNPs in ant..
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Funding Acknowledgements
This work was supported by the Centre for Infectious Diseases and Microbiology-Public Health; however, the funding body had no role in the design of the study, collection, analysis and interpretation of data and in the writing of the manuscript.