Journal article

The development of Clostridium difficile genetic systems

N Minton, G Carter, M Herbert, T O'Keeffe, D Purdy, M Elmore, A Ostrowski, O Pennington, I Davis

Anaerobe | ELSEVIER SCI LTD | Published : 2004

Abstract

Clostridum difficile is a major cause of healthcare-associated disease in the western world, and is particularly prominent in the elderly. Its incidence is rising concomitant with increasing longevity. More effective countermeasures are required. However, the pathogenesis of C. difficile infection is poorly understood. The lack of effective genetic tools is a principal reason for this ignorance. For many years, the only tools available for the transfer of genes into C. difficile have been conjugative transposons, such as Tn916, delivered via filter mating from Bacillus subtilis donors. They insert into a preferred site within the genome. Therefore, they may not be employed for classical muta..

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University of Melbourne Researchers