In vitro tracking and intracellular protein distribution in immunology
Kajal Zibaei, Sarah M Russell
Immunology and Cell Biology | NATURE PUBLISHING GROUP | Published : 2017
New imaging techniques have enabled major advances in understanding how immune reactions are initiated, coordinated and controlled. Imaging methods, which were previously mostly descriptive and supplementary to more quantitative approaches, have now reached sufficient precision and throughput that they are becoming integral to almost all aspects of immunology research. Imaging methodologies that increase the resolution and sensitivity of detection, alongside an ever-expanding range of fluorescent reporters of molecular and cellular activity, and vastly improved analysis methods, have all facilitated this transformation. In this review, we will discuss how advances in imaging are changing the..View full abstract
We thank Ye Chen and Mirren Charnley for helpful comments on the review. SMR is supported by the National Health and Medical Research Council.