Improving HIV surveillance in Victoria: the role of the "detuned" enzyme immunoassay.
Rebecca J Guy, Alan M Breschkin, Cathy M Keenan, Mike G Catton, Adam M Enriquez, Margaret E Hellard
J Acquir Immune Defic Syndr | Published : 2005
Between 1999 and 2000, new diagnoses of HIV in Victoria (Australia) rose by 41%, from 140 to 197. In this time period, sera from new HIV diagnoses were tested using the Organon Teknika "detuned" enzyme immunoassay (EIA). We compared the results of the detuned EIA with incident infections defined by surveillance (on the basis of a previous negative or indeterminate HIV test and/or a seroconversion illness within the 12 months preceding HIV diagnosis). Of 317 specimens, 97 (31%) incident infections and 114 (36%) recent infections were detected using surveillance and detuned EIA, respectively. The detuned assay misclassified 11 cases with AIDS and 2 cases with CD4 counts < or = 200 micro3 (prob..View full abstract