Journal article
Utility of circulating serum miRNAs as biomarkers of early cartilage degeneration in animal models of post-traumatic osteoarthritis and inflammatory arthritis
LHW Kung, S Zaki, V Ravi, L Rowley, MM Smith, KM Bell, JF Bateman, CB Little
Osteoarthritis and Cartilage | ELSEVIER SCI LTD | Published : 2017
Abstract
Objective The purpose of this study was to determine if serum microRNA (miRNA) signatures were biomarkers of early cartilage degeneration in preclinical mouse models of post-traumatic osteoarthritis (OA) and inflammatory arthritis. Methods Cartilage degeneration was induced in 10–12 week old male C57BL6 mice by destabilization of the medial meniscus (DMM) or intra-articular injection of methylated-bovine-serum-albumin (AIA), with sham-operated or saline-injected control animals (n = 6/treatment/time). Total serum RNA and knee joints were isolated at 1, 4 and 16 weeks post-induction. Cartilage degeneration was scored histologically. Serum miRNA expression profiling was performed using Agilent..
View full abstractGrants
Awarded by National Health and Medical Research Council of Australia Project
Funding Acknowledgements
Supported by the National Health and Medical Research Council of Australia Project Grants APP112204 and APP1045890, an Australian Orthopaedic Association Research Foundation Project Grant, Perpetual Trustees through The Hillcrest Foundation Project Grant, and by the Victorian Government's Operational Infrastructure Support Program.