Journal article

A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.

Anthony JM Verberne

Bioengineering (Basel) | Published : 2016

Abstract

Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The cur..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council