Journal article
Role of NADPH Oxidase-4 in human endothelial progenitor cells
NY Hakami, AK Ranjan, AA Hardikar, GJ Dusting, HM Peshavariya
Frontiers in Physiology | FRONTIERS MEDIA SA | Published : 2017
Abstract
Introduction: Endothelial progenitor cells (EPCs) display a unique ability to promote angiogenesis and restore endothelial function in injured blood vessels. NADPH oxidase 4 (NOX4)-derived hydrogen peroxide (H2O2) serves as a signaling molecule and promotes endothelial cell proliferation and migration as well as protecting against cell death. However, the role of NOX4 in EPC function is not completely understood. Methods: EPCs were isolated from human saphenous vein and mammary artery discarded during bypass surgery. NOX4 gene and protein expression in EPCs were measured by real time-PCR and Western blot analysis respectively. NOX4 gene expression was inhibited using an adenoviral vector exp..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This study was supported by the National Heart Foundation of Australia Grant-in-Aid (G 12M 6726) and Postdoctoral Fellowship (HP; PF 11M 6093). GD is the recipient of a National Health and Medical Research Council Research Fellowship (#1003113). NH was supported by King Abdulaziz University, Jeddah, SA. Authors are thankful to National Center for Cell Science, Pune, India for providing EPC. The Centre for Eye Research Australia receives Operational Infrastructure Support from the Victorian Government.