The T-cell Receptor Repertoire Influences the Tumor Microenvironment and Is Associated with Survival in Aggressive B-cell Lymphoma
Colm Keane, Clare Gould, Kimberley Jones, David Hamm, Dipti Talaulikar, Jonathan Ellis, Frank Vari, Simone Birch, Erica Han, Peter Wood, Kim-Anh Le-Cao, Michael R Green, Pauline Crooks, Sanjiv Jain, Josh Tobin, Raymond J Steptoe, Maher K Gandhi
Clinical Cancer Research | AMER ASSOC CANCER RESEARCH | Published : 2017
Purpose: To investigate the relationship between the intra-tumoral T-cell receptor (TCR) repertoire and the tumor microenvironment (TME) in de novo diffuse large B-cell lymphoma (DLBCL) and the impact of TCR on survival.Experimental Design: We performed high-throughput unbiased TCRβ sequencing on a population-based cohort of 92 patients with DLBCL treated with conventional (i.e., non-checkpoint blockade) frontline "R-CHOP" therapy. Key immune checkpoint genes within the TME were digitally quantified by nanoString. The primary endpoints were 4-year overall survival (OS) and progression-free survival (PFS).Results: The TCR repertoire within DLBCL nodes was abnormally narrow relative to non-dis..View full abstract
We acknowledge funding support from the Pathology Queensland Study, Education and Research Committee, the Leukaemia Foundation of Queensland, the Leukaemia Foundation of Australia - Bridgestone Award, Metro South Health Private Practice Trust Fund, and the Princess Alexandra Research Foundation.