Stop codons in the hepatitis B surface proteins are enriched during antiviral therapy and are associated with host cell apoptosis
Danielle Colledge, Sally Soppe, Lilly Yuen, Lucy Selleck, Renae Walsh, Stephen Locarnini, Nadia Warner
VIROLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2017
Premature stop codons in the hepatitis B virus (HBV) surface protein can be associated with nucleos(t)ide analogue resistance due to overlap of the HBV surface and polymerase genes. The aim of this study was to determine the effect of the replication of three common surface stop codon variants on the hepatocyte. Cell lines were transfected with infectious HBV clones encoding surface stop codons rtM204I/sW196*, rtA181T/sW172*, rtV191I/sW182*, and a panel of substitutions in the surface proteins. HBsAg was measured by Western blotting. Proliferation and apoptosis were measured using flow cytometry. All three surface stop codon variants were defective in HBsAg secretion. Cells transfected with ..View full abstract
Awarded by National Health and Medical Research Council (NHMRC)
This work was funded by the National Health and Medical Research Council (NHMRC) (Grant GNT1011024).