Inhibition of hepatitis B virus replication in vivo by nucleoside analogues and siRNA.
Christian Klein, C Thomas Bock, Heiner Wedemeyer, Torsten Wüstefeld, Stephen Locarnini, Hans Peter Dienes, Stefan Kubicka, Michael P Manns, Christian Trautwein
Gastroenterology | Published : 2003
BACKGROUND & AIMS: Hepatitis B virus (HBV) causes acute and chronic infections that may result in severe liver diseases. Animal models to study new treatment options in vivo have several drawbacks. Therefore, we were interested to establish a new small animal model in which HBV replication and especially new treatment options can be studied easily. METHODS: Naked DNA of an HBV replication competent vector was transferred via tail vein into NMRI mice. HBV replication was studied in serum and liver of the animals. HBV replication was modulated by treatment through siRNA and nucleoside analogues. RESULTS: Tail vein transfer of a HBV replication competent construct resulted in expression of HBV-..View full abstract