The half-life of duck hepatitis B virus supercoiled DNA in congenitally infected primary hepatocyte cultures.
GM Civitico, SA Locarnini
Virology | Published : 1994
The transcriptional template for duck hepatitis B virus (DHBV) replication is believed to be the supercoiled covalently closed circular (CCC) molecule. DHBV CCC DNA can be amplified at least 50-fold in acutely and congenitally infected hepatocyte cultures but is normally maintained at a constant copy number in vivo infections. Here we describe experiments to determine the half-life of DHBV CCC DNA in congenitally infected hepatocyte cultures using both direct and indirect labeling of DHBV CCC DNA with the DNA labeling agent 5-bromo 2-deoxyuridine (BrUdR). Direct labeling of DHBV CCC DNA with BrUdR generated a very stable molecule with no calculable half-life. For indirect labeling experiment..View full abstract