Using a multi-biomarker approach to assess the effects of pollution on sand flathead (Platycephalus bassensis) from Port Phillip Bay, Victoria, Australia
Dingkun Fu, Andrew Bridle, Melanie Leef, Marthe Monique Gagnon, Kathryn L Hassell, Barbara F Nowak
MARINE POLLUTION BULLETIN | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2017
Hepatic gene expression and liver histology were examined in sand flathead (Platycephalus bassensis) from six locations in Port Phillip Bay, Victoria, Australia. Four sets of genes including thyroid-related genes (D1, D2, TTR, TRα and TRβ), metal metabolism-related genes (MT, MTF1, TF, Ferritin and FPN1), apoptosis-related genes (Diablo/SMAC1, Diablo/SMAC2 and CYP1A) and an endoplasmic reticulum stress biomarker gene (GRP78) were examined in female flathead using qRT-PCR. TRβ and Diablo/SMAC1 gene expression was significantly up-regulated in fish from all polluted sites compared to those from a reference site. The transcripts of TRα and FPN1 were significantly higher in flathead from Corio B..View full abstract
The authors wish to thank Jarrad Baker, Sara Long, Jessica French, Sherrie Chambers and Rod Watson for fieldwork assistance, and Liz McGrath, Rhianna Boyle and Vin Pettigrove for technical and other assistance. The project was supported by Curtin University, the Centre for Aquatic Pollution Identification and Management (CAPIM) and the Victorian Marine Science Consortium. All fish were collected by hook and line as per general research permit (RP1216) granted by the Department of Environment and Primary Industries (Victoria State Government) and Curtin University Animal Ethics approval number AEC_2015_05.