Astrocyte mGlu2/3-mediated cAMP potentiation is calcium sensitive: Studies in murine neuronal and astrocyte cultures

Abstract

Signal transduction mechanisms of group II metabotropic glutamate receptors (mGlu2/3) remains a matter of some controversy, therefore we sought to gain new insights into its regulation by studying cAMP production in cultured neurons and astrocytes, and by examining inter-relationships of mGlu2/3-induced signalling with cellular calcium and various signalling cascades. mGlu2/3 agonists 2R,4R-4-aminopyrrolidine-2,4-dicarboxylic acid (2R,4R-APDC) and (-)-2-oxa-4-aminobicyclo[3.1.0]hexane-4,6-dicarboxylic acid (LY379268) inhibited 10 μM forskolin-stimulated production of cAMP in murine cortical neurons, striatal neurons and forebrain astrocytes in the absence of extracellular Ca2+. These agonist..