Cholecystokinin-GABA interactions in rodent cortex: analyses of cholecystokinin effects on K( )- and L-glutamate-induced release of [3H]GABA from rat cortical slices and cultured mouse cortical neurones.
YM Hickling, NS Cheung, JA Larm, MS Cowen, A Shulkes, PM Beart
Neurochemistry International | Published : 1997
Neurones of the cerebral cortex immunoreactive for the neuropeptide, cholecystokinin (CCK), also invariably contain GABA. Hence CCK is believed to modulate some aspect of GABAergic synaptic activity. The present study therefore investigated the effects of CCK on basal, K(+)- and L-glutamate-induced release of [3H]GABA from slices of rat neocortex and cultured murine neocortical neurones. Rat neocortical prisms loaded with [3H]GABA (10 nM) were superfused with Krebs-Henseleit buffer and stimulated twice (S1 and S2, 2 min) with K+ (30 mM). Release associated with each stimulus was measured and expressed relative to basal release (R1 and R2). The effects of non-selective and CCKB selective agon..View full abstract