Journal article

Wheat (Triticum aestivum L.) grain proteome response to elevated [CO2] varies between genotypes

Pramesha Madurangi S Arachchige, Ching-Seng Ang, Marc E Nicolas, Joe Panozzo, Glenn Fitzgerald, Naoki Hirotsu, Saman Seneweera



The impact of rising carbon dioxide concentration ([CO2]) in the atmosphere on wheat grain protein concentration and proteome was investigated in this study. Wheat genotypes (H45, SB003, SB062 and Yitpi) were grown in the Australian Grains Free-Air CO2 Enrichment (AGFACE) facility, Horsham, Victoria, Australia under ambient [CO2] (a[CO2], 391 μmol mol−1) and elevated [CO2] (e[CO2], 550 ± 20 μmol mol−1). Grain yield and grain protein concentration were measured. Global grain proteome comparison was carried out using stable isotope dimethyl labelling followed by liquid chromatography - mass spectrometry (LC-MS/MS). Grain yield was significantly increased at e[CO2], whereas protein concentratio..

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Awarded by University of Melbourne

Awarded by Victorian State Government Department of Primary Industries

Funding Acknowledgements

AGFACE facility is jointly run by the University of Melbourne and Victorian State Government Department of Primary Industries (Grant no. DAV00121) with additional funding by the Grains Research and Development Corporation (GRDC), Australian Government Department of Agriculture, and the Australian Research Council (ARC). The authors gratefully acknowledge the contribution of the AGFACE field team lead by Russel Argall (DEPI, Horsham) for field management, and Mahabubur Mollah (DEPI, Horsham) for operating the CO<INF>2</INF> enrichment technology and staff at proteomics facility, Bio 21 Institute for the support extended towards analysis. The authors are grateful to CSIRO (F. Dreccer, S. Chapman) for supplying sufficient amounts of seeds of the CIMMYT lines. Additional technical support by Peter Howie is gratefully acknowledged. Pramesha is supported by a Melbourne International Research Scholarship, a Melbourne International Fee Remission Scholarship and Graduate student research support grant.