Costorage of Enteroendocrine Hormones Evaluated at the Cell and Subcellular Levels in Male Mice
Linda J Fothergill, Brid Callaghan, Billie Hunne, David M Bravo, John B Furness
ENDOCRINOLOGY | OXFORD UNIV PRESS INC | Published : 2017
Recent studies reveal complex patterns of hormone coexpression within enteroendocrine cells (EECs), contrary to the traditional view that gut hormones are expressed individually in EECs. Moreover, different hormones have been found in separate subcellular vesicles. However, detailed analysis of relative expression of multiple hormones has not been made. Subcellular studies have been confined to peptide hormones, and have not included the indolamine 5-hydroxytryptamine (5-HT) or the neuroendocrine protein chromogranin A (CgA). In the present work, coexpression of 5-HT, CgA, secretin, cholecystokinin (CCK), ghrelin, and glucagonlike peptide (GLP)-1 in mouse duodenum was quantified at a cellula..View full abstract
This work was supported by an Australian Government Research Training Program Scholarship to L.J.F.