Journal article

Identifying cytomegalovirus complications using the quantiferon-CMV assay after allogeneic hematopoietic stem cell transplantation

MK Yong, PU Cameron, M Slavin, CO Morrissey, K Bergin, A Spencer, D Ritchie, AC Cheng, A Samri, G Carcelain, B Autran, SR Lewin

Journal of Infectious Diseases | OXFORD UNIV PRESS INC | Published : 2017

Abstract

Background. A simple test to identify recovery of CMV-specific T-cell immunity following hematopoietic stem cell transplantation (HSCT) could assist clinicians in managing CMV-related complications. Methods. In an observational, multicenter, prospective study of 94 HSCT recipients we evaluated CMV-specific T-cell immunity at baseline, 3, 6, 9, and 12 months after transplant using the Quantiferon-CMV, an enzyme-linked immunosorbent spot assay (ELISpot), and intracellular cytokine staining. Results. At 3 months after HSCT, participants who developed CMV disease (n = 8) compared with CMV reactivation (n = 26) or spontaneous viral control (n = 25) had significantly lower CD8+ T-cell production o..

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