Journal article

Stability of Dried Blood Spots for HIV-1 Drug Resistance Analysis

Anna C Hearps, Claire E Ryan, Lisa M Morris, Megan M Plate, Vicki Greengrass, Suzanne M Crowe

Current HIV Research | BENTHAM SCIENCE PUBL LTD | Published : 2010

Abstract

The wide scale application of dried blood spots (DBS) as a collection tool for low-cost HIV drug resistance testing requires a greater understanding of the accuracy of DBS for genotype analysis and the stability of DBS under various environmental conditions. Analysis of a 50microl DBS via a single amplicon, nested PCR-based in-house assay (the Burnet genotyping assay) showed an average nucleotide concordance of 98.9% with plasma samples, although only 65% of nucleotide mixtures detected in plasma were also detected within DBS. The analysis of three DBS resulted in the detection of a greater number of nucleotide mixtures (72 and 109 mixtures detected within one and three DBS, respectively, n=..

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