Journal article

C57BL/6J mouse apolipoprotein A2 gene is deterministic for apnea

Carl B Gillombardo, Rebecca Darrah, Thomas E Dick, Michael Moore, Nathan Kong, Michael J Decker, Fang Han, Motoo Yamauchi, Mathias Dutschmann, Sausan Azzam, Kingman P Strohl

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | ELSEVIER SCIENCE BV | Published : 2017

Abstract

RATIONALE: Brainstem apolipoprotein AII (apoa2) mRNA expression correlates with apnea in breathing present in the adult C57Bl/6J (B6) sleep apnea model. OBJECTIVES: To test the hypothesis that the B6 apoa2 gene contributes to the trait, we performed plethysmographic testing in apoa2 knock out (KO: -/-) mice, an in situ brainstem-spinal cord preparation comparing KO to WT (+/+) mice, and B6xDBA recombinant inbred strains (RISs). MEASUREMENTS AND MAIN RESULTS: Apoa2 WT do, but KO and heterozygote (+/-) mice do not exhibit apnea during post-hypoxic breathing, measured in vivo. In the in situ model, pauses and instability in fictive phrenic bursting are substantially reduced in KO vs. WT prepara..

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Grants

Awarded by Merit Award from the VA Research Service


Awarded by NIH


Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE


Awarded by NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE


Awarded by Veterans Affairs


Awarded by Grants-in-Aid for Scientific Research


Funding Acknowledgements

We wish to thank Gjinovefa (Gina) Kola for her diligence and skill with the in situ preparation. This work is supported by Merit Award from the VA Research Service I01BX001464 to KPS, and by the NIH (R21NS052452 to KPS and T32HL07913 to SMA). We thank the Louis Stokes Cleveland DVA Medical Center Animal Resource Center for the assistance in the breeding and care of animals.