Journal article
An iron-oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase
EP Tchesnokov, AS Faponle, CG Davies, MG Quesne, R Turner, M Fellner, RJ Souness, SM Wilbanks, SP De Visser, GNL Jameson
Chemical Communications | ROYAL SOC CHEMISTRY | Published : 2016
DOI: 10.1039/c6cc03904a
Abstract
Cysteine dioxygenase is a key enzyme in the breakdown of cysteine, but its mechanism remains controversial. A combination of spectroscopic and computational studies provides the first evidence of a short-lived intermediate in the catalytic cycle. The intermediate decays within 20 ms and has absorption maxima at 500 and 640 nm.
Grants
Funding Acknowledgements
GNLJ thanks the Marsden Fund of the Royal Society of New Zealand for funding. EPT thanks the Canadian Institutes of Health Research for a postdoctoral fellowship. RJS was the recipient of a TEC Top Achiever Doctoral Scholarship. SdV thanks the National Service of Computational Chemistry Software (NSCCS) for cpu time. ASF and MGQ thank the Tertiary Education Trust Fund and the BBSRC for studentships. B. Zhang, C. Pollock, J. M. Bollinger Jr. and C. Krebs of Penn State are thanked for freeze-quench studies down to 10 ms.