Journal article

Recombinant Escherichia coli biotin synthase is a [2Fe-2S](2 ) protein in whole cells

MM Cosper, GNL Jameson, MK Eidsness, BH Huynh, MK Johnson

FEBS LETTERS | WILEY | Published : 2002

Abstract

EPR and Mössbauer spectroscopies have been used to determine the type and properties of the iron-sulfur clusters present in homologously expressed recombinant Escherichia coli BioB in whole cells prior to purification. Difference EPR spectra of samples of whole cells from a strain over-expressing E. coli BioB and a strain containing the same plasmid but without the bioB insertion showed an axial S=1/2 resonance that was attributed to the [2Fe-2S](+) cluster of the E. coli iron-sulfur cluster assembly 2Fe ferredoxin, based on principal g-values, linewidths and relaxation behavior. Comparison of the Mössbauer spectra of whole cells with and without the bioB insertion revealed that the E. coli ..

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University of Melbourne Researchers