Journal article

Recombinant Escherichia coli biotin synthase is a [2Fe-2S](2 ) protein in whole cells

MM Cosper, GNL Jameson, MK Eidsness, BH Huynh, MK Johnson

FEBS LETTERS | WILEY | Published : 2002


EPR and Mössbauer spectroscopies have been used to determine the type and properties of the iron-sulfur clusters present in homologously expressed recombinant Escherichia coli BioB in whole cells prior to purification. Difference EPR spectra of samples of whole cells from a strain over-expressing E. coli BioB and a strain containing the same plasmid but without the bioB insertion showed an axial S=1/2 resonance that was attributed to the [2Fe-2S](+) cluster of the E. coli iron-sulfur cluster assembly 2Fe ferredoxin, based on principal g-values, linewidths and relaxation behavior. Comparison of the Mössbauer spectra of whole cells with and without the bioB insertion revealed that the E. coli ..

View full abstract

University of Melbourne Researchers