Journal article

Kinetic evidence that cysteine reacts with dopaminoquinone via reversible adduct formation to yield 5-cysteinyl-dopamine: an important precursor of neuromelanin

GNL Jameson, J Zhang, RF Jameson, W Linert

ORGANIC & BIOMOLECULAR CHEMISTRY | ROYAL SOC CHEMISTRY | Published : 2004

Abstract

The reaction of cysteine (cys) with dopaminoquinone (DQ) to form (mainly) 5-cysteinyl-dopamine (5-cys-DA) is of interest because it is known to play a role in the production of melanin in the mammalian brain. To gain insight into this important reaction, an in vitro detailed kinetic study was undertaken. It has been established that cys reacts with DQ via the initial reversible formation of an intermediate adduct or complex and that this adduct then decomposes to form 5-cys-DA. A little 2-cys-DA, is almost certainly formed at the same time but its presence could not be kinetically investigated. Clarification of the kinetic data was aided by following the reaction of DQ with a cys analogue, m..

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