Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis
Amy TY Yeung, Christine Hale, Amy H Lee, Erin E Gill, Wendy Bushell, David Parry-Smith, David Goulding, Derek Pickard, Theodoros Roumeliotis, Jyoti Choudhary, Nick Thomson, William C Skarnes, Gordon Dougan, Robert EW Hancock
Nature Communications | NATURE PUBLISHING GROUP | Published : 2017
Awarded by Canadian Institutes for Health Research (CIHR)
This work was supported by The Wellcome Trust. REWH would like to acknowledge funding from the Canadian Institutes for Health Research (CIHR, funding reference number MOP-363437) for the work reported here and personal support from the Canada Research Chair program and University of British Columbia Killam Professorship. We thank Dr Ian Clarke for providing us with the C. trachomatis strains Sweden F plasmid minus (SWFP -) and SWFP - transformed with plasmid pGFP::SW2. We thank Theresa Feltwell, Louise Ellison and Dr Helena Smith for sharing Chlamydia propagation protocols. We thank Drs Rey Carabeo and Tristan Thwaites for teaching us how to perform density gradient purification of Chlamydia. We thank Gareth Griffiths and Katie Andrews for help with genotyping.