Journal article
Human alkaline phosphatase dephosphorylates microbial products and is elevated in preterm neonates with a history of late-onset sepsis
M Pettengill, JD Matute, M Tresenriter, J Hibbert, D Burgner, P Richmond, JL Millán, A Ozonoff, T Strunk, A Currie, O Levy
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2017
Abstract
Background A host defense function for Alkaline phosphatases (ALPs) is suggested by the contribution of intestinal ALP to detoxifying bacterial lipopolysaccharide (endotoxin) in animal models in vivo and the elevation of ALP activity following treatment of human cells with inflammatory stimuli in vitro. However the activity of ALP in human plasma (primarily tissue-nonspecific ALP; TNAP) on lipopolysaccharide and other microbial products has not been assessed, nor has its expression been studied in preterm newborns, a vulnerable population at high risk of sepsis. In this context, the aim of our study was to characterize the activity of TNAP on Toll-like receptor (TLR) agonists and assess the ..
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Awarded by National Institute of Dental and Craniofacial Research
Funding Acknowledgements
This study in OL's lab was supported by Global Health (OPPGH5284) and Grand Challenges Explorations (OPP1035192) awards from the Bill & Melinda Gates Foundation http://www.gatesfoundation.org/) and by NIH grant 1R01AI100135-01 and its administrative supplement 3R01AI067353-05S1 (https://www.nih.gov/). MP was supported by NIH Training Grant T32 HD055148. DB is supported by an NHMRC Senior Research Fellowship (APP1064629) (https://www.nhmrc.gov.au/) and an honorary NHFA Future Leaders Fellowship (100026) (http:// heartfoundation.org.au/). JLM is supported by NIH grant R01 DE12889. Samples collection from infants at King Edward Memorial Hospital was funded by an NHMRC project grant (APP37601100). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.