Prostaglandin D-2 Regulates SOX9 Nuclear Translocation during Gonadal Sex Determination in Tammar Wallaby, Macropus eugenii
Yu Chen, Hongshi Yu, Andrew J Pask, Geoff Shaw, Marilyn B Renfree
Sexual Development | Karger Publishers | Published : 2017
Sex determination and sexual differentiation pathways are highly conserved between marsupials and eutherians. There are 2 different pathways of prostaglandin D2 (PGD2) synthesis: prostaglandin D synthase (PTGDS) and haematopoietic prostaglandin D synthase (HPGDS). PGD2 regulates the subcellular localization of SOX9 during gonadal sexual differentiation. To investigate the function of PGD2 in the tammar gonad, we cultured undifferentiated male gonads in the presence of the HPGDS inhibitor HQL-79 and female gonads with exogenous PGD2 to mimic activation of the PTGDS-PGD2 pathway. Tammar PTGDS and HPGDS have only 50% similarity with mouse and human orthologues, but functional domains are conser..View full abstract
We thank Scott Brownlees and all members of the wallaby research group for assistance with the tammars. We thank Prof. Asao Fujiyama for the transcriptome data. This study was supported by a project grant from the National Health and Medical Research Council of Australia.