Mouse Saliva Inhibits Transit of Influenza Virus to the Lower Respiratory Tract by Efficiently Blocking Influenza Virus Neuraminidase Activity
Brad Gilbertson, Wy Ching Ng, Simon Crawford, Jenny L McKimm-Breschkin, Lorena E Brown
JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2017
We previously identified a novel inhibitor of influenza virus in mouse saliva that halts the progression of susceptible viruses from the upper to the lower respiratory tract of mice in vivo and neutralizes viral infectivity in MDCK cells. Here, we investigated the viral target of the salivary inhibitor by using reverse genetics to create hybrid viruses with some surface proteins derived from an inhibitor-sensitive strain and others from an inhibitor-resistant strain. These viruses demonstrated that the origin of the viral neuraminidase (NA), but not the hemagglutinin or matrix protein, was the determinant of susceptibility to the inhibitor. Comparison of the NA sequences of a panel of H3N2 v..View full abstract
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Awarded by National Health and Medical Research Council of Australia
This study was funded by Project Grant 509281 and Program Grant 567122 from the National Health and Medical Research Council of Australia. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.