Journal article

The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X.

DR Bentley, P Deloukas, A Dunham, L French, SG Gregory, SJ Humphray, AJ Mungall, MT Ross, NP Carter, I Dunham, CE Scott, KJ Ashcroft, AL Atkinson, K Aubin, DM Beare, G Bethel, N Brady, JC Brook, DC Burford, WD Burrill Show all

Nature | Published : 2001

Abstract

We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenc..

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