Journal article

Human Immunodeficiency Virus Persistence and T-Cell Activation in Blood, Rectal, and Lymph Node Tissue in Human Immunodeficiency Virus-Infected Individuals Receiving Suppressive Antiretroviral Therapy

Gabriela Khoury, Remi Fromentin, Ajantha Solomon, Wendy Hartogensis, Marisela Killian, Rebecca Hoh, Ma Somsouk, Peter W Hunt, Valerie Girling, Elizabeth Sinclair, Peter Bacchetti, Jenny L Anderson, Frederick M Hecht, Steven G Deeks, Paul U Cameron, Nicolas Chomont, Sharon R Lewin

JOURNAL OF INFECTIOUS DISEASES | OXFORD UNIV PRESS INC | Published : 2017

Abstract

Background: Immune activation and inflammation remain elevated in human immunodeficiency virus (HIV)-infected individuals receiving antiretroviral therapy (ART) and may contribute to HIV persistence. Methods: Using flow cytometry expression of CD38, HLA-DR and PD-1 were measured in blood (n = 48), lymph node (LN; n = 9), and rectal tissue (n = 17) from virally suppressed individuals. Total and integrated HIV DNA, 2-LTR circles, and cell-associated unspliced HIV RNA were quantified. Results: CD4+ T cells from rectal tissue had a higher frequency of integrated HIV DNA compared with blood (4.26 fold-change in DNA; 95% confidence interval [CI] = 2.61-7.00; P < .001) and LN (2.32 fold-change in D..

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Grants

Awarded by National Institutes of Health Delaney AIDS Research Enterprise


Awarded by National Health and Medical Research Council of Australia (NHMRC) Dora Lush biomedical post-graduate scholarship


Awarded by amfAR fellowship


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by the National Institutes of Health Delaney AIDS Research Enterprise (U19 AI096109). G. K is a recipient of a National Health and Medical Research Council of Australia (NHMRC) Dora Lush biomedical post-graduate scholarship (579719). R. F. is supported by an amfAR fellowship (108264-51-RFRL). S. R. L is an NHMRC practitioner fellow.