Journal article

An automated, multiplex-tandem PCR platform for the diagnosis of gastrointestinal nematode infections in cattle: An Australian-European validation study

Florian Roeber, Ebrahim Bani Hassan, Philip Skuce, Alison Morrison, Edwin Claerebout, Stijn Casaert, David R Homer, Simon Fireston, Mark Stevenson, Lee Smith, John Larsen



Detecting the genera and species of gastrointestinal (GI) nematode infections in faecal samples obtained from cattle requires the incubation of faeces ('larval culture') followed by identification of the third-stage larvae that are harvested after 10-14days. Substantial research in the development of PCR-based methods for the rapid and specific identification GI nematodes has been conducted for small ruminants, whilst only few such assays have been developed for cattle. In the present paper we describe the development of an automated, robotic PCR platform for the detection and genus and/or species-specific identification of GI nematodes from bovine faecal samples. This test was then validate..

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Funding Acknowledgements

This study was funded by Merial Animal Health. The authors would like to acknowledge the financial support of the Scottish Government's Rural and Environment Science and Analytical Services (RESAS) to PS & AAM, Moredun Research Institute. SMF is supported by an Australian Research Council Discovery Early Career Researcher Award. Aspects of this research were conducted on the Victorian Life Sciences Computation Initiative's Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government, Australia. The authors would also like to thank Emeritus Professor Ian Beveridge and Dr. Norman Anderson for their constructive comments regarding the prevalence of different parasite species in Australia. Supply of nematode eggs and larvae through VHR laboratory is gratefully acknowledged.