Journal article

Genetic diversity and relatedness estimates for captive barramundi (Lates calcarifer, Bloch) broodstock informs efforts to form a base population for selective breeding

Shannon R Loughnan, Carolyn Smith-Keune, Dean R Jerry, Luciano B Beheregaray, Nicholas A Robinson

AQUACULTURE RESEARCH | WILEY | Published : 2016

Abstract

Aquaculture of barramundi or Asian seabass (Lates calcarifer) is growing in both Australia and Southeast Asia and there is substantial interest to improve production efficiency through selective breeding. The establishment of a large and genetically diverse base population is a prerequisite for a sustainable and long‐term productive breeding program. Before selective breeding programs can begin for Australian barramundi it is important to assess the overall genetic diversity of current captive broodstock populations. To address this question, 407 captive barramundi broodstock from eight separate Australian broodstock populations were genotyped using 16 polymorphic microsatellite DNA marker..

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University of Melbourne Researchers

Grants

Awarded by Australian Barramundi Farmers Association


Funding Acknowledgements

The authors would like to thank the Australian Seafood CRC, the Fisheries Research and Development Corporation and the Australian Barramundi Farmers Association for funding this study (project 2009/730). This research was conducted as part of S.L. PhD dissertation research (animal ethics approval project number E345), supported by an Australian Seafood CRC bursary and Flinders University AJ & IM Naylon PhD scholarship. All molecular work was carried out within the Molecular Ecology and Evolution Laboratory (MEEL) at James Cook University and utilized multiplex marker conditions developed or modified from published conditions within the Aquaculture Genetics Research Group (Smith-Keune and Jerry). We are grateful to the hatcheries involved in this study, for allowing access to the samples and providing background information on the broodstock.