Journal article
Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection
Sweta Das, Chiranjibi Chhottaray, Kanta Das Mahapatra, Jatindra Nath Saha, Matthew Baranski, Nicholas Robinson, PK Sahoo
MOLECULAR BIOLOGY REPORTS | SPRINGER | Published : 2014
Abstract
A total of 137,629 contigs generated via de novo transcriptome assembly from resistant and susceptible lines of rohu (first generation) raised against aeromoniasis were further analyzed in terms of defence-related genes. Out of 1,939 contigs showing homology to genes involved in immune processes, 1,866 were further categorised into different functional subgroups. Comparative analysis revealed five genes for the first time in any carp species out of which apolipoprotein h, septin 4 isoform 3 and septin isoform cra_c were identified for the first time in fish. Differential expression analysis of ten genes viz., heat shock proteins (Hsps) (Hsp30, Hsp70 and Hsp90), serum lectin isoform 1 (SLI1),..
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Funding Acknowledgements
Financial support received from Department of Biotechnology, Government of India, New Delhi for Indian workers and Research Council of Norway for Norwegian workers is gratefully acknowledged. The authors are thankful to the Director, Central Institute of Freshwater Aquaculture, Bhubaneswar, India for providing necessary facilities during this study.