Journal article

The Development of a High Density Linkage Map for Black Tiger Shrimp (Penaeus monodon) Based on cSNPs

Matthew Baranski, Gopalapillay Gopikrishna, Nicholas A Robinson, Vinaya Kumar Katneni, Mudagandur S Shekhar, Jayakani Shanmugakarthik, Sarangapani Jothivel, Chavali Gopal, Pitchaiyappan Ravichandran, Matthew Kent, Mariann Arnyasi, Alphis G Ponniah

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2014

Abstract

Transcriptome sequencing using Illumina RNA-seq was performed on populations of black tiger shrimp from India. Samples were collected from (i) four landing centres around the east coastline (EC) of India, (ii) survivors of a severe WSSV infection during pond culture (SUR) and (iii) the Andaman Islands (AI) in the Bay of Bengal. Equal quantities of purified total RNA from homogenates of hepatopancreas, muscle, nervous tissue, intestinal tract, heart, gonad, gills, pleopod and lymphoid organs were combined to create AI, EC and SUR pools for RNA sequencing. De novo transcriptome assembly resulted in 136,223 contigs (minimum size 100 base pairs, bp) with a total length 61 Mb, an average length o..

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University of Melbourne Researchers

Grants

Awarded by Indian Council of Agricultural Research of India


Funding Acknowledgements

The authors are thankful to the Norwegian Research Council, Department of Biotechnology of India and Indian Council of Agricultural Research of India for funding this project (project 183833). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.