Journal article

Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling

M Baranski, M Rourke, S Loughnan, B Hayes, C Austin, N Robinson

ANIMAL GENETICS | WILEY | Published : 2008


The objective of this study was to identify QTL for growth rate in the blacklip abalone Haliotis rubra using selective DNA pooling. Three full-sibling families of H. rubra derived from crosses of wild broodstock were used. DNA was extracted from the largest and smallest 10% of progeny and combined into two pools for each phenotypic tail. The DNA pools were typed with 139 microsatellites, and markers showing significant differences between the peak height ratios of alleles inherited from the parents were individually genotyped and analysed by interval mapping. A strong correlation (r = 0.94, P < 0.001) was found between the t-values from the analysis of pools and the t-values from the analysi..

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University of Melbourne Researchers


Awarded by Australian Fisheries Research and Development Corporation

Funding Acknowledgements

The authors sincerely thank Mark Gervis and Anton Krsinich from Southern Ocean Mariculture who maintained and raised the abalone families used in this study. Nathan O'Mahoney and Steve Leporati assisted in the collection of phenotypic data. Funding for this research was provided by the Australian Fisheries Research and Development Corporation (project number 2002/202) and Fisheries Victoria. Matthew Baranski was supported by an Australia Postgraduate Award through Deakin University, with additional funding from the Department of Primary Industries.