Journal article

Quantitative determination of metal and metalloid spatial distribution in hydrated and fresh roots of cowpea using synchrotron-based X-ray fluorescence microscopy

Peng Wang, Neal W Menzies, Enzo Lombi, Brigid A McKenna, Martin D de Jonge, Erica Donner, F Pax C Blamey, Chris G Ryan, David J Paterson, Daryl L Howard, Simon A James, Peter M Kopittke

SCIENCE OF THE TOTAL ENVIRONMENT | ELSEVIER SCIENCE BV | Published : 2013

Abstract

Many metals and metalloids, jointly termed metal(loid)s, are toxic to plants even at low levels. This has limited the study of their uptake, distribution, and modes of action in plant roots grown at physiologically relevant concentrations. Synchrotron-based X-ray fluorescence microscopy was used to examine metal(loid)s in hydrated cowpea (Vigna unguiculata L.) roots exposed to Zn(II), Ni(II), Mn(II), Cu(II), Hg(II), Se(IV), Se(VI), As(III), or As(V). Development of a mathematical model enabled in situ quantitative determination of their distribution in root tissues. The binding strength of metals influenced the extent of their movement through the root cylinder, which influenced the toxic ef..

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University of Melbourne Researchers

Grants

Awarded by Australian Synchrotron, Melbourne, Victoria, Australia


Awarded by Australian Research Council (ARC) DECRA


Awarded by ARC


Funding Acknowledgements

A substantial part of this research received support from the Australian Synchrotron, Melbourne, Victoria, Australia to use the XFM (AS112/XFM/3570 and AS121/XFM/4502) beam line. Support was also provided to Dr. Wang as a recipient of an Australian Research Council (ARC) DECRA (DE130100943) and to Dr. Kopittke as a recipient of an ARC Future Fellowship (FT120100277).