An assessment of genetic relationships between members of the phytophthora megasperma complex and phytophthora vignae using molecular markers

Abstract

Genetic relationships between Phytophthora megasperma f. sp. glycinea (Pmg) and morphologically similar taxa, P. megasperma f. sp. medieaginis (Pmm), P. megasperma f. sp. trifolii (Pmt), P. megasperma from Douglas Fir (PmDF) and asparagus (PmAS) and Phytophthora vignae, were explored by restriction fragment length polymorphism (RFLP) analysis of nuclear DNA using random genomic multi-copy, cDNA, and ribosomal DNA probes as well as random amplified polymorphic DNA (RAPDs) and RFLP analysis of ribosomal intergenic spacer regions amplified by the polymerase chain reaction (PCR). Each method detected large differences between these taxa and P. megasperma f. sp. glycinea. P. vignae was more close..