Assessing the development of oseltamivir and zanamivir resistance in A(H5N1) influenza viruses using a ferret model
Aeron C Hurt, Sue Lowther, Deborah Middleton, Ian G Barr
ANTIVIRAL RESEARCH | ELSEVIER | Published : 2010
Using an in vivo ferret model, we investigated the development of resistance to oseltamivir and zanamivir for two different influenza A(H5N1) viruses (A/Vietnam/1203/2004, haemagglutinin phylogenetic clade 1, and A/Chicken/Laos/26/2006, haemagglutinin phylogenetic clade 2.3) by treating the animals with doses equivalent either to the recommended human treatment dose or a range of sub-optimal drug doses. No resistance was observed in oseltamivir-treated ferrets, but analysis of nasal washes from zanamivir-treated ferrets infected with influenza A/Vietnam/1203/2004 revealed one viral isolate (from a ferret receiving the highest dose of zanamivir, 1.0mg/kg twice daily) with a zanamivir IC(50) t..View full abstract
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Awarded by Australian National Health and Medical Research Council
Supported by Australian National Health and Medical Research Council award 400595. We also acknowledge the expert technical assistance of Tim Hancock and Jessica Klippel. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health and Ageing.