Journal article

Lactosylated Glycogen Nanoparticles for Targeting Prostate Cancer Cells

Quinn A Besford, Marcin Wojnilowicz, Tomoya Suma, Nadja Bertleff-Zieschang, Frank Caruso, Francesca Cayalieri

ACS APPLIED MATERIALS & INTERFACES | AMER CHEMICAL SOC | Published : 2017

Abstract

Glyconanoparticles that exhibit multivalent binding to lectins are desirable for molecular recognition and therapeutic applications. Herein we explore the use of glycogen nanoparticles as a biosourced glycoscaffold for engineering multivalent glyconanoparticles. Glycogen nanoparticles, a naturally occurring highly branched polymer of glucose, was functionalized with lactose, achieved through copper(I)-catalyzed alkyne-azide cycloaddition chemistry, for targeted interaction with lectins ex situ and on prostate cancer cells. The lactosylated glycogen, which contains terminal β-galactoside moieties, is termed galacto-glycogen (GG), and is found to interact strongly with peanut agglutinin (PNA),..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by Australian Laureate Fellowship schemes


Awarded by ARC Centre of Excellence in Convergent Bio-Nano Science and Technology


Funding Acknowledgements

We thank Dr. David Stapleton for helpful discussions and Dr. Christine Browne for AFM assistance. This work was supported by the Australian Research Council (ARC) under the Future Fellowship (F. Cavalieri, FT140100873) and Australian Laureate Fellowship (F. Caruso, FL120100030) schemes. This work was also supported by a University of Melbourne Establishment Grant ( F. Cavalieri). This research was conducted by the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). This work was performed in part at the Materials characterization and Fabrication Platform (MCFP) at the University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF).