The effect of short-term overfeeding on serum lipids in healthy humans
Leonie K Heilbronn, Adelle CF Coster, Lesley V Campbell, Jerry R Greenfield, Kylie Lange, Michael J Christopher, Peter J Meikle, Dorit Samocha-Bonet
OBESITY | WILEY | Published : 2013
OBJECTIVES: While chronic obesity is associated with alterations in circulating glycerolipids, sphingolipids and plasmalogens, the effects of short-term overfeeding in humans are unclear. DESIGN AND METHODS: Healthy individuals (n = 40) were overfed by 1,250 kcal day(-1) for 28 days. Insulin sensitivity (hyperinsulinemic-euglycemic clamp), abdominal fat distribution and serum lipidomics (mass spectrometry) were assessed. RESULTS: Overfeeding increased liver fat, insulin resistance, serum C-reactive protein and urinary F2-isoprostanes. HDL increased (11% ± 2%, P < 0.001) while LDL, triglycerides and nonesterified fatty acids were unchanged. Three hundred and thirty three serum lipids were det..View full abstract
This work was supported by funding from the National Health and Medical Research Council (NHMRC) of Australia, the OIS Program of the Victorian Government, Australia, Diabetes Australia Research Trust and the Sachdev Foundation. PJM is supported by a NHMRC Senior Research Fellowship, LKH by Australian Research Council (ARC) Future Fellowship and DS-B by Australian Diabetes Society (ADS) Fellowship.