Journal article
Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid
D Fraher, A Sanigorski, NA Mellett, PJ Meikle, AJ Sinclair, Y Gibert
Cell Reports | CELL PRESS | Published : 2016
Open access
Abstract
The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processe..
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Funding Acknowledgements
The authors would like to thank the Deakin University zebrafish facility for providing excellent husbandry care. Y.G. is supported by the Centre for Molecular and Medical Research and a Central Research Grant Scheme at Deakin University.