Journal article
Caveolin-1 plays a critical role in the differentiation of monocytes into macrophages
Y Fu, XL Moore, MKS Lee, MA Fernández-Rojo, MO Parat, RG Parton, PJ Meikle, D Sviridov, JPF Chin-Dusting
Arteriosclerosis Thrombosis and Vascular Biology | LIPPINCOTT WILLIAMS & WILKINS | Published : 2012
Abstract
Objective-Monocyte to macrophage differentiation is an essential step in atherogenesis. The structure protein of caveolae, caveolin-1, is increased in primary monocytes after its adhesion to endothelium. We explore the hypothesis that caveolin-1 plays a role in monocyte differentiation to macrophages. Methods and Results-Both phorbol myristate acetate-induced THP-1 and colony-stimulating factor-induced primary monocyte differentiation was associated with an increase in cellular caveolin-1 expression. Overexpression of caveolin-1 by transfection increased macrophage surface markers and inflammatory genes, whereas caveolin-1 knockdown by small interfering RNA or knockout reduced these. Also, c..
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Funding Acknowledgements
Y. Fu is supported by an overseas-based training fellowship from the Australian National Health and Medical Research Council (NHMRC). D. Sviridov. is a Senior Research Fellow of NHMRC and R.G. Parton., an NHMRC Australia Fellow. This work is supported, in part, by the Victorian Government's Operational Infrastructure Support Program.