Circulating microparticles generate and transport monomeric C-reactive protein in patients with myocardial infarction
Jonathon Habersberger, Frederik Strang, Amelie Scheichl, Nay Htun, Nicole Bassler, Ruusu-Maaria Merivirta, Philipp Diehl, Guy Krippner, Peter Meikle, Steffen U Eisenhardt, Ian Meredith, Karlheinz Peter
CARDIOVASCULAR RESEARCH | OXFORD UNIV PRESS | Published : 2012
AIMS: Elevated serum C-reactive protein (CRP) following myocardial infarction (MI) is associated with poor outcomes. Although animal studies have indicated a direct pathogenic role of CRP, the mechanism underlying this remains elusive. Dissociation of pentameric CRP (pCRP) into pro-inflammatory monomers (mCRP) may directly link CRP to inflammation. We investigated whether cellular microparticles (MPs) can convert pCRP to mCRP and transport mCRP following MI. METHODS AND RESULTS: MPs enriched in lysophosphatidylcholine were obtained from cell cultures and patient whole-blood samples collected following acute MI and control groups. Samples were analysed by native western blotting and flow cyto..View full abstract
This work was supported by a project grant of the National Health and Medical Research Council (NHMRC) of Australia. J.H. was supported by a joint scholarship of the NHMRC and Heart Foundation. F.S. was supported by a scholarship from the University of Schleswig-Holstein, Campus Lubeck, Germany. K.P. was supported by an Australian Research Council Future Fellowship.