Journal article

INTERLEUKIN 10 ANTIOXIDANT EFFECT DECREASES LEUKOCYTES/ENDOTHELIAL INTERACTION INDUCED BY TUMOR NECROSIS FACTOR alpha

Olivier Huet, Elisabette Laemmel, Yi Fu, Laurent Dupic, Andrea Aprico, Karen L Andrews, Shirley L Moore, Anatole Harrois, Peter L Meikle, Eric Vicaut, Jaye PF Chin-Dusting, Jacques Duranteau

SHOCK | LIPPINCOTT WILLIAMS & WILKINS | Published : 2013

Abstract

Little is known about the endothelial mechanisms involved in the anti-inflammatory effects of interleukin 10 (IL-10). The goal of this study was to evaluate the effects of IL-10 on endothelial oxidative stress and endothelial inflammation induced by tumor necrosis factor α (TNF-α). Production of reactive oxygen species (ROS) in perfused human umbilical vein endothelial cells (HUVECs) was studied by fluorescent microscopy using dichlorodihydrofluorescein diacetate. Tumor necrosis factor α (1 ng/mL) was added to the perfusion medium in the absence and presence of IL-10 (1 ng/mL). The role of phosphatidylinositol 3-kinase (PI3-kinase) was assessed using wortmannin and LY 2940002 (inhibitors of ..

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