Journal article
Measurement of tissue azithromycin levels in self-collected vaginal swabs post treatment using liquid chromatography and tandem mass spectrometry (LC-MS/MS)
LA Vodstrcil, TWT Rupasinghe, FYS Kong, D Tull, K Worthington, MY Chen, WM Huston, P Timms, MJ McConville, CK Fairley, CS Bradshaw, SN Tabrizi, JS Hocking
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2017
Abstract
Background Azithromycin is recommended for the treatment of uncomplicated urogenital chlamydia infection although the standard 1gram dose sometimes fails to eradicate the infection (treatment failure). One hypothesis proposed for treatment failure has been insufficient levels of the antibiotic at the site of infection. We developed an assay using liquid chromatography and tandem mass spectrometry (LC-MS/MS) to measure azithromycin concentration in high-vaginal swabs and monitor how concentration changes over time following routine azithromycin treatment. Methods Azithromycin concentrations were measured in two groups of women either within the first 24h of taking a 1g dose (N = 11) or over 9..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This study was funded by the National Health and Medical Research Council of Australia (project grant 1023239) and J.S. Hocking supported by a NHMRC Senior Research Fellowship (1042907).