Journal article

DEFINITION OF BRUCELLA-A AND BRUCELLA-M EPITOPES BY MONOCLONAL TYPING REAGENTS AND SYNTHETIC OLIGOSACCHARIDES

DR BUNDLE, JW CHERWONOGRODZKY, MAJ GIDNEY, PJ MEIKLE, MB PERRY, T PETERS

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 1989

Abstract

The paradigm that Brucella A and M epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies. Monoclonal antibodies were generated to the M antigen of Brucella melitensis 16M. Chemically defined lipopolysaccharides and O polysaccharides from Brucella abortus 1119-3, B. melitensis 16M, and Yersinia enterocolitica O:9 were used to dissect the binding profiles of the B. melitensis antibodies and an additional set of antibodies available from a B. abortus fusion experiment. Binding specificities were rationalized in terms of prototype A- and M-antigen structures, an interpretation support..

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