Journal article

HUMAN ACETYL-COENZYME A-ALPHA-GLUCOSAMINIDE N-ACETYLTRANSFERASE - KINETIC CHARACTERIZATION AND MECHANISTIC INTERPRETATION

PJ MEIKLE, AM WHITTLE, JJ HOPWOOD

Biochemical Journal | PORTLAND PRESS | Published : 1995

Abstract

Acetyl-CoA: alpha-glucosaminide N-acetyltransferase (N-acetyltransferase) is an integral lysosomal membrane protein which catalyses the transfer of acetyl groups from acetyl-CoA on to the terminal glucosamine in heparin and heparan sulphate chains within the lysosome. In vitro, the enzyme is capable of acetylating a number of mono- and oligo-saccharides derived from heparin, provided that a non-reducing terminal glucosamine is present. We have prepared highly enriched lysosomal membrane fractions from human placenta by a combination of differential centrifugation and density-gradient centrifugation in Percoll. This preparation was used to investigate the kinetics of the enzyme with three ace..

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