Journal article
Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status
D Mittag, AI Proietto, T Loudovaris, SI Mannering, D Vremec, K Shortman, L Wu, LC Harrison
Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2011
Abstract
Mouse dendritic cells (DC) have been extensively studied in various tissues, especially spleen, and they comprise subsets with distinct developmental origins, surface phenotypes, and functions. Considerably less is known about human DC due to their rarity in blood and inaccessibility of other human tissues. The study of DC in human blood has revealed four subsets distinct in phenotype and function. In this study, we describe four equivalent DC subsets in human spleen obtained from deceased organ donors. We identify three conventional DC subsets characterized by surface expression of CD1b/c, CD141, and CD16, and one plasmacytoid DC subset characterized by CD304 expression. Human DC subsets in..
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Funding Acknowledgements
L.C.H, L.W., and K.S. are supported by National Health and Medical Research Council of Australia (NHMRC) research fellowships. This work was also supported by a project grant from the Juvenile Diabetes Research Foundation, NHMRC program grants (to L.C.H. and K.S.), an NHMRC project grant (to L.W.), the Cancer Council of Victoria (to L.W.), and a Victorian State Government Operational Infrastructure Support grant.