Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1
Diana Mittag, Amrita Sran, Kasey S Chan, Martin P Boland, Esther Bandala-Sanchez, Olivier Huet, William Xu, Rosemary L Sparrow
TRANSFUSION | WILEY | Published : 2015
BACKGROUND: Biochemical changes induced in red blood cells (RBCs) during storage may impair their function upon transfusion. Transfusion-associated stresses may further amplify storage lesion effects including increased phosphatidylserine (PS) exposure at the RBC membrane, microparticle (MP) release, and adhesion to endothelial cells (ECs). RBC stress susceptibility in vitro was investigated in relation to storage time and additive solution. STUDY DESIGN AND METHODS: Leukoreduced whole blood donations (n = 18) were paired, mixed, and resplit before separating the RBCs for storage in saline-adenine-glucose-mannitol (SAGM) or AS-1. Samples were taken after 3, 21, or 35 days. For oxidative stre..View full abstract
Awarded by NIH
Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
This work was funded by NIH Grant 1R01 HL095470-01A1. We acknowledge the Australian government, which fully fund the Australian Red Cross Blood Service for the provision of blood products and services to the Australian community.