Journal article
acDCs enhance human antigen-specific T-cell responses
E Martinuzzi, G Afonso, MC Gagnerault, G Naselli, D Mittag, B Combadière, C Boitard, N Chaput, L Zitvogel, LC Harrison, R Mallone
Blood | AMER SOC HEMATOLOGY | Published : 2011
Abstract
Detection of human Ag-specific T cells is limited by sensitivity and blood requirements. As dendritic cells (DCs) can potently stimulate T cells, we hypothesized that their induction in PBMCs in situ could link Ag processing and presentation to Ag-specific T-cell activation. To this end, unfractionated PBMCs (fresh or frozen) or whole blood were incubated for 48 hours with protein or peptide Ag together with different DC-activating agents to rapidly and sequentially induce, pulse, and mature DCs. DC activation was therefore lined up withAg recognition by neighboring T cells, thus telescoping the sequential steps of T-cell activation. Efficient processing of protein Ags made prior knowledge o..
View full abstractGrants
Awarded by Juvenile Diabetes Research Foundation (JDRF)
Awarded by National Health and Medical Research Council (NHMRC) of Australia
Funding Acknowledgements
This study was supported by grants from the Juvenile Diabetes Research Foundation (JDRF grant 1-2008-106), the European Foundation for the Study of Diabetes (EFSD/JDRF/Novo Nordisk European Program in Type 1 Diabetes Research 2007), the Inserm Program National de Recherche sur le Diabete 2007, the Fondation Recherche Medicale (grant Installation Nouvelle Equipe), and the Ile-de-France CODDIM (grant Soutien aux Jeunes Equipes) to R. M. and by program (516700) and infrastructure (361646) grants from the National Health and Medical Research Council (NHMRC) of Australia, a Victorian State Government Operational Infrastructure Support Grant, and a grant from the Diabetes Vaccine Development Center, Australia, to L. C. H. R. M. is an Inserm Avenir Investigator and L. C. H. is a Senior Principal Research Fellow of the NHMRC. E. M. was the recipient of a postdoctoral fellowship from the Fondation Recherche Medicale and of an EFSD Albert Renold travel fellowship.